magnification factor =
= (magnification of the objective lens) * (magnification of any attachment if any, else 1)
NOTE: The magnification factor does not include the power of the ocular eyepiece.
radius of the microscopic field of view in millimeters =
= (eye piece field-of-view number) / (2 * (magnification factor))
where:
• The eyepiece field-of-view number is written on the side of the eyepiece (for example: 20, 22 or 25).
area in microscopic field of view in square millimeters =
= (
π * ((radius)^2))
Alternatively the radius can be directly measured using a special slide with an etched ruler marked in microns.
|
Microscope
|
Field of View Number for Standard Eyepiece
|
Area in square mm with 40x Objective
|
|
Leica
|
20
|
0.20
|
|
Nikon
|
22
|
0.24
|
|
Olympus BH2
|
20
|
0.20
|
|
Olympus BX40
|
22
|
0.24
|
|
Zeiss
|
20
|
0.20
|
from Table 2, page 936.
number of fields required to examine a given area in square millimeters =
= (area in square millimeters to examine) / (area of a microscopic field in square millimeters)
