Beer's Law relates the absorbance of a solution with the concentration of the test compound under specific conditions.
absorbance =
= (proportionality constant) * (length of light path in centimeters) * (concentration of the absorbing compound)
The proportionality constant is typically the molar absorptivity (episolon) for the compound in the solvent under specific conditions and temperature.
molar absorptivity =
= (absorbance) / ((light path in centimeters) * (concentration in mol/L))
concentration of compound in solution =
= ((absorbance) * (molecular weight of compound in grams)) / ((molar absorptivity) * (length of light path))
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Specialty: Clinical Laboratory
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