The average retention factor for a chromatographic system is the instantaneous isocratic retention factor for a peak traveling halfway through the column. It can be calculated based on the system conditions.
Parameters:
(1) gradient time in minutes
(2) flow rate in mL per minute
(3) low end of gradient range in percent
(4) high end of gradient range in percent
(5) volume of the column in mL
(6) characteristic factor for the analyte (default value is given as 5)
difference in the gradient range as a decimal fraction =
= ((high end of gradient range in percent) - (low end of gradient range)) / 100
average retention factor =
= (gradient time) * (flow rate) / ((different in gradient range) * (volume of column) * (analyte factor))
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