A glycerol density gradient can help separate cells and subcellular components based on density.
Molecular weight of glycerol: 92.09
Density of glycerol: 1.25 g/mL
Parameter: aqueous glycerol solution as percent weight by weight, as a decimal fraction from 0 to 0.60.
percent weight by weight as decimal fraction =
= (grams of solute) / (total weight of solution)
density of a glycerol solution at a given percent (weight for weight) as a decimal fraction =
= (0.04508 * ((percent)^2)) + (0.2323 * (percent)) + 0.9979
refractive index of a glycerol solution at a given percent (weight for weight) as a decimal fraction =
= (0.03929 * ((percent)^2)) + (0.1160 * (percent)) + 1.333
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Specialty: Clinical Laboratory
