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Description

A sucrose density gradient can help separate cells and subcellular components based on density.


 

Molecular weight of sucrose: 342.3

 

Parameter: aqueous sucrose solution as percent weight by weight, as a number from 0 to 60

 

percent weight by weight =

= (grams of solute) / (total weight of solution) * 100%

 

density of a sucrose solution at a given percent (weight for weight) =

= (0.000017 * ((percent)^2)) + (0.00375 * (percent)) + 0.9988

 

refractive index of a sucrose solution at a given percent (weight for weight) =

= (0.000007086 * ((percent)^2)) + (0.001380 * (percent)) + 1.333

 


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