Immunoperoxidase staining can be helpful in differentiating between spindle cell tumors of the gastrointestinal tract.
Differential diagnosis:
(1) GI stromal tumor (GIST)
(2) smooth muscle tumor (leiomyoma)
(3) schwannoma
(4) fibromatosis
Immunoperoxidase panel:
(1) anti-KIT (CD117)
(2) CD34
(3) smooth muscle actin (SMA)
(4) desmin
(5) S-100
|
Antibody |
GIST |
smooth muscle tumor |
schwannoma |
fibromatosis |
|
anti-KIT |
positive |
negative |
negative |
negative (see comment) |
|
anti-CD34 |
60—70% positive |
10 – 15% positive |
positive in Antoni B areas |
rare |
|
anti-actin |
30-40% positive |
positive |
negative |
positive |
|
anti-desmin |
very rarely positive |
positive |
negative |
rare cells |
|
anti-S-100 |
5+% |
rarely positive |
positive |
negative |
where:
• A few authors report that fibromatoses may be positive on anti-KIT staining.
Limitations:
• Staining using immunoperoxidase methods may have false positives and negatives, depending on the quality of the antibodies and the method used.
• Considerable inter-observer variability can occur.
Specialty: Hematology Oncology, Surgery, general, Gastroenterology
ICD-10: ,
