From the number of red cells and the intra-erythrocytic concentration of an analyte one could adjust the results for the hemolyzed specimen.
Methods:
(1) plasma hemoglobin and mean cell hemoglobin (MCH)
(2) change in absolute RBC count (requires whole blood)
(3) ratio of plasma hemoglobin and blood hemoglobin
Parameters:
(1) plasma hemoglobin in mg/dL
(2) MCH in pg per RBC
(3) hemoglobin in g/dL
(4) RBC count in 10^6/µL or 10^12/L
number of RBCs hemolyzed based on MCH =
= (X plasma hemoglobin in mg/dL) / (Y MCH in pg/cell) =
= X/Y * 10^10 cells per L
= X/Y * 0.01 * 10^12 cells per L
number of RBCs hemolyzed based on change in RBC count =
= (RBC count of a nonhemolyzed specimen) – (RBC count of hemolyzed specimen)
number of RBCs hemolyzed based on ratio of hemoglobins =
= (nonhemolyzed RBC count) * (X plasma hemoglobin in mg/dL) / (Y hemoglobin in g/dL) =
= (nonhemolyzed RBC count) * 0.001 * X/Y
