HBSS (Hank's Balanced Salt Solution) is an important solution for handling cells and tissues since it maintains the proper osmotic and pH balance.
Component |
Molecular Weight |
NaCl |
58.44 |
KCl |
74.55 |
CaCl2 |
110.98 |
MgSO4•7H2O |
246.47 |
MgCl2•6H2O |
203.303 |
Na2HPO4•2H2O |
177.99 |
KH2PO4 |
136.086 |
D-glucose |
180.156 |
NaHCO3 |
84.01 |
Component |
Grams per liter |
Molarity |
NaCl |
8 |
0.14 |
KCl |
0.400 |
0.005 |
CaCl2 |
0.140 |
0.001 |
MgSO4•7H2O |
0.100 |
0.0004 |
MgCl2•6H2O |
0.100 |
0.0005 |
Na2HPO4•2H2O |
0.060 |
0.0003 |
KH2PO4 |
0.060 |
0.0004 |
D-glucose |
1 |
0.006 |
NaHCO3 |
0.350 |
0.004 |
A pH indicator may be added, resulting in a colored solution.
Component |
Molecular Weight |
NaCl |
58.44 |
KCl |
74.55 |
CaCl2 |
110.98 |
MgSO4•7H2O |
246.47 |
MgCl2•6H2O |
203.303 |
Na2HPO4•2H2O |
177.99 |
KH2PO4 |
136.086 |
D-glucose |
180.156 |
NaHCO3 |
84.01 |
Component |
Grams per liter |
Molarity |
NaCl |
8 |
0.14 |
KCl |
0.400 |
0.005 |
CaCl2 |
0.140 |
0.001 |
MgSO4•7H2O |
0.100 |
0.0004 |
MgCl2•6H2O |
0.100 |
0.0005 |
Na2HPO4•2H2O |
0.060 |
0.0003 |
KH2PO4 |
0.060 |
0.0004 |
D-glucose |
1 |
0.006 |
NaHCO3 |
0.350 |
0.004 |
A pH indicator may be added, resulting in a colored solution.
Specialty: Clinical Laboratory