The Human Blood Index (HBI) is the proportion of mosquito blood meals derived from humans. It can be used to estimate the risk of malaria transmission in a community.
Analytical method: PCR using primers to mitochondrial DNA from various animals targeted by mosquitoes (human, cow, pig, goat, dog, etc)
Parameters:
(1) total number of mosquitoes collected
(2) total number of collected mosquitoes with evidence of blood meal
(3) total number of collected mosquitoes with evidence of human blood
crude HBI =
= (number of mosquitoes with human blood) / (total number of mosquitoes collected)
Variations on the index:
(1) ratio based on mosquitoes with blood meals
(2) ratio based on mosquitoes with unidentified blood meals excluded
(3) ratios for each species of msoquito
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